Title:Two-Photon Lithographic Patterning of DNA-Coated Single-Microparticle Surfaces

Author(s):Huang, FJ (Huang, Fujian); Zhang, J (Zhang, Juan); Li, T (Li, Tao); Duan, RL (Duan, Ruilin); Xia, F (Xia, Fan); Willner, I (Willner, Itamar)

Addresses:Huang, Fujian,China Univ Geosci, Fac Mat Sci & Chem, Minist Educ, Engn Res Ctr Nanogeomat, Wuhan 430074, Hubei, Peoples R China; Xia, F (reprint author),China Univ Geosci, Fac Mat Sci & Chem, Minist Educ, Engn Res Ctr Nanogeomat, Wuhan 430074, Hubei, Peoples R China.

Source:NANO LETTERS Volume:19 Issue:1 Pages:618-625

DOI:10.1021/acs.nanolett.8b04975

Published:JAN 2019

Abstract:The spatially defined functionalization of microparticles with asymmetric shape-controlled nucleic acid patterns is a major challenge in materials science. The asymmetric patterning of micro particles is important to allow the controlled fabrication of crystalline lattices or controlled aggregates of microparticles. We present the combination of two-photon lithography and photocleavable o-nitro-benzylphosphate ester nucleic acid coating-modified microparticles as a versatile means to asymmetrically pattern single microparticle surfaces. The two-photon patterning of microparticles with predesigned nucleic acid structures of different sizes (700 nm to 2.8 mu m) and shapes (circles, rings, triangles, and squares) are demonstrated. In addition, complex patterned domains consisting of two different asymmetric nucleic acid domains are fabricated by the controlled Z-positioning of the microparticles in respect to the two-photon irradiation sources. In addition, the two-photon lithographic patterning of the photocleavable DNA coating allows the generation of functional nucleic acid domains for the photostimulated activation of the catalytic hybridization assembly (CHA) of branched nucleic acid structures on single microparticles.

Full Text from Publisher:https://pubs.acs.org.ccindex.cn/doi/10.1021/acs.nanolett.8b04975